| 1 | - 1Emma Kern12
- 2Brooke Puttick10
- 3Avery McKeska10
- 4Lola Pisanek11
| | Amanda-Clearcreek | 54.44 | | 2 | 10 |
| 2 | - 1Abrial Johnson12
- 2Keegan Dunham12
- 3Kiera Whiting9
- 4Ryanne Moore10
| | Piketon | 55.89 | | 2 | 8 |
| 3 | - 1Breanna Clark10
- 2Grace Woodward11
- 3Kaycee Fannin10
- 4Amber Blevins12
| | Wheelersburg | 56.39 | | 2 | 6 |
| 4 | - 1Kamryn Sowers12
- 2Olivia Ferrell10
- 3Mia Wilburn10
- 4Jacey Jones9
| | Adena | 56.58 | | 2 | 5 |
| 5 | - 1Nev Bolin11
- 2Avery Nathan12
- 3Angela Blanton10
- 4Kylee Lawson11
| | Waverly | 56.62 | | 1 | 4 |
| 6 | - 1Reise Grant11
- 2Fiona Gillow10
- 3Hadley Matthews12
- 4Brea Alley12
| | Zane Trace | 57.29 | | 1 | 3 |
| 7 | - 1Kierstin Runkle12
- 2Kaylee Curtis10
- 3Emma Parker10
- 4Jayanna Patete9
| | Westfall | 57.64 | | 1 | 2 |
| 8 | - 1Dejah Thomas10
- 2Roni Lytle11
- 3Meloney Sims9
- 4Lili Ary10
| | Huntington Ross | 58.29 | | 2 | 1 |
| 9 | - 1Emma Mosier10
- 2Gemma Bell9
- 3Addyson Stewart10
- 4Leila Kiser12
| | Southeastern | 1:02.15 | | | 1 |