| 1 | - 1Estie Nehls8
- 2Amiya Brooks8
- 3Mindy Wright8
- 4Kaylee Laubacher8
| | Oak Harbor | 54.48 | | 2 | 10 |
| 2 | - 1Grace Clay8
- 2Avery Evans8
- 3Riley Harkelroad8
- 4Ava Hicks8
| | McCormick | 55.33 | | 1 | 8 |
| 3 | - 1Kaylyn Hoyt8
- 2Kinze Dauch8
- 3Presley Speer8
- 4Layla Price8
| | Bellevue | 55.42 | | 2 | 6 |
| 4 | - 1Lily Bohn8
- 2Hailey Miller8
- 3Rhylan Brown8
- 4Macie Berg8
| | Margaretta | 55.56 | | 2 | 5 |
| 5 | - 1Reese Schaefer8
- 2Jaxson Alexander7
- 3Lyllah Williams8
- 4Baela Link8
| | Briar | 55.62 | | 2 | 4 |
| 6 | - 1Payton Smith8
- 2Loreli Clapp8
- 3Lea Berger7
- 4Charlie Polachek8
| | Seneca East | 56.19 | | 2 | 3 |
| 7 | - 1Liv Darovich8
- 2Kayla Naseman7
- 3Evie Stowers8
- 4Londynn Clifton8
| | Norwalk | 57.22 | | 2 | 2 |
| 8 | - 1Amaya Kimbrough8
- 2Rory Propri8
- 3Riley Revette7
- 4Aubrianna McKenna7
| | Midview | 57.25 | | 1 | 1 |
| 9 | - 1Kaydence Stuckey8
- 2Kennedy Lawrence7
- 3Leighton Tanner7
- 4Olivia Webb7
| | Tiffin MS | 57.30 | | | 2 |
| 10 | - 1Adrienne Albrecht7
- 2Camille Prater8
- 3Sadie Pollard7
- 4Brynn Keys8
| | Fremont | 57.59 | | | 2 |
| 11 | - 1Charlotte Dauch8
- 2Bria Hector8
- 3Brynn Blackburn8
- 4Brinn Root8
| | McPherson | 58.49 | | | 1 |
| 12 | - 1Kara Valkenhaus7
- 2Charlee Whiting8
- 3Berkeley Mizener8
- 4Trinity Silverwood8
| | Port Clinton | 58.50 | | | 1 |
| 13 | - 1Kaley Coleman7
- 2August Hines8
- 3Kennady Robertson8
- 4Quinn Horvath8
| | Edison | 1:00.14 | | | 1 |