| 1 | - 1Kamryn Sowers12
- 2Olivia Ferrell10
- 3Mia Wilburn10
- 4Jacey Smith11
| | Adena | 55.80 | | | 10 |
| 2 | - 1Sophia Stauffer12
- 2Chloe Kinzer9
- 3Dejiah Crowder10
- 4Izzy Garnes9
| | Paint Valley | 56.21 | | | 8 |
| 3 | - 1Nev Bolin11
- 2Sadie Royster11
- 3Leah Walters11
- 4Caris Risner12
| | Waverly | 56.75 | | | 6 |
| 4 | - 1Abrial Johnson12
- 2Keegan Dunham12
- 3Kiera Whiting9
- 4Ryanne Moore10
| | Piketon | 57.47 | | | 5 |
| 5 | - 1Marideth Barrow10
- 2Chloe Wood9
- 3Vivian Grimes11
- 4Addalynn Perkins9
| | Winc. Eastern | 59.09 | | | 4 |
| 6 | - 1Emma Mosier10
- 2Gemma Bell9
- 3Kaydence Cordial11
- 4Leila Kiser12
| | Southeastern | 1:04.30 | | | 3 |
| 7 | - 1Hayleigh Donahue10
- 2Evalin May9
- 3Keeley Gamble10
- 4Emileigh Willis9
| | Huntington Ross | 1:06.60 | | | 2 |
| 8 | - 1Hyalee Shields11
- 2KiAnna McKinley9
- 3Lily Howard10
- 4Madee Henderson11
| | Peebles | 1:09.74 | | | 1 |