| 1 | - 1Lilah Anderson9
- 2Hannah Kempton12
- 3Sophie Hanes9
- 4Grace McBee12
| | Unioto | 53.82 | | 2 | 10 |
| 2 | - 1Aspen Ruggles10
- 2Taylor Cron9
- 3Lexie Claxon10
- 4Jackie Pendleton12
| | Minford | 56.38 | | 2 | 8 |
| 3 | - 1Nev Bolin11
- 2Sadie Royster11
- 3Rachel Remy12
- 4Caris Risner12
| | Waverly | 56.97 | | 2 | 6 |
| 4 | - 1Abrial Johnson12
- 2Keegan Dunham12
- 3Kiera Whiting9
- 4Ryanne Moore10
| | Piketon | 57.27 | | 2 | 5 |
| 5 | - 1Mia Rose9
- 2Ava Smith12
- 3Izzy Boyd12
- 4Ella Thompson10
| | Valley | 57.59 | | 1 | 4 |
| 6 | - 1Liberty Ward10
- 2Brynna Detty10
- 3Cylie Weaver12
- 4Audrey Nolen12
| | Bea. Eastern | 59.03 | | 2 | 3 |
| 7 | - 1Reise Grant11
- 2Alexcis Barrows10
- 3Hadley Matthews12
- 4Brea Alley12
| | Zane Trace | 1:00.06 | | 1 | 2 |
| 8 | - 1Sarah Wilburn12
- 2Hannah Phipps12
- 3Bri Jones11
- 4Madison Bender10
| | Northwest | 1:00.10 | | 2 | 1 |
| 9 | - 1Dejah Thomas10
- 2Roni Lytle11
- 3Leighton Keiser9
- 4Lili Ary10
| | Huntington Ross | 1:01.67 | | | 1 |
| 10 | - 1Emma Mosier10
- 2Gemma Bell9
- 3Kaydence Cordial11
- 4Leila Kiser12
| | Southeastern | 1:04.02 | | | 1 |
| 11 | - 1Angel Stacey9
- 2Lilly Carver10
- 3Zoe Woodyard11
- 4Alexa Mears9
| | South Webster | 1:07.12 | | | 1 |