| 1 | - 1Brinnley Ward9
- 2Braelynn Shupe10
- 3Marlee Laudermilt10
- 4Adyson Fields12
| | Meigs | 53.86 | | 2 | 10 |
| 2 | - 1Carsen McKinniss9
- 2Sydney Carpenter11
- 3Braelyn Brennan11
- 4Gabby Palm11
| | Jackson | 54.08 | | 2 | 8 |
| 3 | - 1Kayden Bean10
- 2Abby Sanders12
- 3Lily Ryder10
- 4Kalli Jarvis10
| | Alexander | 54.46 | | 2 | 6 |
| 4 | - 1Alexis Carter10
- 2Cloey McCarty11
- 3Taylor Barnes11
- 4Illyana Simmons9
| | Gallia Academy | 54.47 | | 1 | 5 |
| 5 | - 1Addison Browning10
- 2Hayley Edwards11
- 3Hennessey Morrison10
- 4Anna Cadle12
| | Bid. River Valley | 54.92 | | 2 | 4 |
| 6 | - 1Alyvia Portier10
- 2Myla Moore9
- 3Macy Vore12
- 4Abby Weber12
| | Trimble | 55.33 | | 2 | 3 |
| 7 | - 1Aspen Ruggles10
- 2Taylor Cron9
- 3Lexie Claxon10
- 4Jackie Pendleton12
| | Minford | 55.98 | | 1 | 2 |
| 8 | - 1Cadynce Brennan10
- 2Hannah Petersen11
- 3Jaylee King9
- 4Emmy Glispie9
| | Wellston | 57.34 | | 1 | 1 |
| 9 | - 1Katie Caldwell10
- 2Emily Shockey12
- 3Nevaeh Camp9
- 4Payton Kranyik10
| | Southern | 1:02.44 | | | 1 |
| 10 | - 1Moriah Myers10
- 2Emma Shaver10
- 3Danika Collins9
- 4Halley Lear11
| | Gallia Academy B | 1:03.70 | | | 1 |