| 1 | - 1Aspen Ruggles10
- 2Taylor Cron9
- 3Lexie Claxon10
- 4Jackie Pendleton12
| | Minford | 54.51 | | 2 | 10 |
| 2 | - 1McKenzie Bailey12
- 2Abigail Miller11
- 3Makenzie Carter9
- 4Layla Dawes9
| | Clay | 54.88 | | 2 | 8 |
| 3 | - 1Addison Kennard10
- 2Grace Woodward11
- 3Kaycee Fannin10
- 4Breanna Clark10
| | Wheelersburg | 55.74 | | 2 | 6 |
| 4 | - 1Nev Bolin11
- 2Sadie Royster11
- 3Rachel Remy12
- 4Caris Risner12
| | Waverly | 56.19 | | 2 | 5 |
| 5 | - 1Sarah Wilburn12
- 2Nakera Hoople9
- 3Bri Jones11
- 4Madison Bender10
| | Northwest | 57.56 | | 1 | 4 |
| 6 | - 1Liberty Ward10
- 2Brynna Detty10
- 3Cylie Weaver12
- 4Olivia Logan11
| | Bea. Eastern | 58.19 | | 2 | 3 |
| 7 | - 1Addison Blizzard10
- 2Emily Butler10
- 3Kailen Dingus9
- 4Matti Hayslip10
| | Green | 58.67 | | 1 | 2 |
| 8 | - 1Mya Smith11
- 2Janecca Marsh9
- 3Bella Mustain9
- 4Kaelyn Tabor10
| | Glenwood | 59.31 | | 1 | 1 |
| 9 | - 1Kailynn Wiedbrauk9
- 2Hailey Carver10
- 3Marrissa Ginn12
- 4Shaley Boggs10
| | Port. West | 59.44 | | | 1 |
| 10 | - 1Kylie Rawlins9
- 2Lilly Carver10
- 3Zoe Woodyard11
- 4Bryleigh Grant9
| | South Webster | 1:02.17 | | | 1 |
| 11 | - 1Jocelyn Carter10
- 2Maliyah Feltner10
- 3Haley Blevins10
- 4Natali Hoskins10
| | Sciot. Community | 1:02.75 | | | 1 |