| 1 | - 1Emily Laker10
- 2Kora Zuber9
- 3Hudsyn Bagley9
- 4Amelia Graham11
| | Antwerp | 53.06 | | 2 | 10 |
| 2 | - 1Brynn Coil9
- 2Nepthalie Miller10
- 3Paige Schwinnen9
- 4Nakiyah Kimmett11
| | Del. Jefferson | 53.23 | | 2 | 8 |
| 3 | - 1Jazlyn Sheets11
- 2Gretchen Schlacter9
- 3Lanie Sheets11
- 4Brooklyn Michel11
| | Ayersville | 53.48 | | 2 | 6 |
| 4 | - 1Carigan Treesh9
- 2Ava Dowd10
- 3Luca Bontrager9
- 4Danica Kessel11
| | Lakewood Park Christian | 54.34 | | 2 | 4 |
| 5 | - 1Karsyn Troth9
- 2Kenzie Ogle9
- 3Peyton Sennebogen9
- 4Lilly Shepherd9
| | Wayne Trace | 55.75 | | 2 | 2 |
| 6 | - 1Aubrey Hall9
- 2Aleena Ankney12
- 3Eowyn Blanchard12
- 4Ella Schaulfele9
| | Tinora | 56.43 | | 1 | 1 |
| 7 | - 1Cali Adams11
- 2Camryn Almester11
- 3Leah Gladieux9
- 4Mariah Clark11
| | Rossford | 56.84 | | | 2 |
| 8 | - 1Lydia VanScoder12
- 2Katherine Green9
- 3Lilah Pascute10
- 4Angelina Crawford12
| | Hicksville | 57.03 | | | 1 |
| 9 | - 1Marley Moyer12
- 2Lexie Hay9
- 3Lloa Mountz12
- 4Grace Ripke11
| | Edon | 57.69 | | | 1 |
| 10 | - 1Makayla Guy9
- 2Grace Froelich11
- 3Anastasia Rodrigues9
- 4Aubrey Clemens10
| | Stryker | 57.93 | | | 1 |
| 11 | - 1Clara Collins10
- 2Heidi Meyer12
- 3Elle Huard9
- 4Keira Myers12
| | Edgerton | 58.29 | | | 1 |
| 12 | - 1Olivia Miles9
- 2Addison Arend9
- 3Alayna White9
- 4Claire Miller12
| | Paulding | 58.89 | | | 1 |