| 1 | - 1Sophia Stauffer12
- 2Faith Proehl9
- 3Dejiah Crowder10
- 4Izzy Garnes9
| | Paint Valley | 53.94 | | 2 | 10 |
| 2 | - 1Emma Kern12
- 2Brooke Puttick10
- 3Avery McKeska10
- 4Lola Pisanek11
| | Amanda-Clearcreek | 54.54 | | 2 | 8 |
| 3 | - 1Lilah Anderson9
- 2Addison Coxson9
- 3Sophie Hanes9
- 4Abbie Proctor9
| | Unioto | 54.62 | | 1 | 6 |
| 4 | - 1Kamryn Sowers12
- 2Olivia Ferrell10
- 3Mia Wilburn10
- 4Jacey Smith11
| | Adena | 54.74 | | 2 | 5 |
| 5 | - 1Lydia Mootispaw11
- 2Jasmine Bishop9
- 3Cara Dean9
- 4Eva Bennett9
| | Washington | 55.09 | | 2 | 4 |
| 6 | - 1Nev Bolin11
- 2Sadie Royster11
- 3Rachel Remy12
- 4Kylee Lawson11
| | Waverly | 55.76 | | 2 | 3 |
| 7 | - 1Abrial Johnson12
- 2Keegan Dunham12
- 3Kiera Whiting9
- 4Ryanne Moore10
| | Piketon | 56.57 | | 1 | 2 |
| 8 | - 1Reise Grant11
- 2Alexcis Barrows10
- 3Hadley Matthews12
- 4Brea Alley12
| | Zane Trace | 57.74 | | 1 | 1 |
| 9 | - 1Dejah Thomas10
- 2Roni Lytle11
- 3Austyn Oliver11
- 4Lili Ary10
| | Huntington Ross | 59.82 | | | 1 |
| 10 | - 1Emma Mosier10
- 2Gemma Bell9
- 3Kaydence Cordial11
- 4Leila Kiser12
| | Southeastern | 1:01.50 | | | 1 |